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Index > Protein center > Il18(Gene name) > Rat
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  • Il18 (Gene name),
  • Interleukin-18 (Protein name ),  IL18_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (1)
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  • Gene name:
    Il18(Igif);
    Protein name:
    Interleukin-18(IL-18);
    Alternative:
    Interleukin-1 gamma(IL-1 gamma);Interferon gamma-inducing factor(IFN-gamma-inducing factor);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    194
    Sequence:
    50:
    MAAMSEEGSC | VNFKEMMFID | NTLYLIPEDN | GDLESDHFGR | LHCTTAVIRS | 
    100:
    INDQVLFVDK | RNPPVFEDMP | DIDRTANESQ | TRLIIYMYKD | SEVRGLAVTL | 
    150:
    SVKDGRMSTL | SCKNKIISFE | EMNPPENIDD | IKSDLIFFQK | RVPGHNKMEF | 
    194:
    ESSLYEGHFL | ACQKEDDAFK | LVLKRKDENG | DKSVMFTLTN | LHQS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    KEGG:
    UniGene:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat IL-18
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    ELISA Kit for Rat IL-18
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    E0064r
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    ELISA Kit for Rat IL-18
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    E0064m
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    ELISA Kit for Rat IL-18
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    96T
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    ELISA Kit for Rat IL-18
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    E0064d
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    ELISA Kit for Rat IL-18
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    E0064b
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    96T
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    ELISA Kit for Rat IL-18
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    E0064c
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    CLIA Kit for Rat IL-18
    Cat.:
    U0064b
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    96T
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    CLIA Kit for Rat IL-18
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    U0064d
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    96T
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    CLIA Kit for Rat IL-18
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    96T
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    CLIA Kit for Rat IL-18
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    U0064m
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    Packing:
    96T
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    CLIA Kit for Rat IL-18
    Cat.:
    U0064r
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    MSDS:
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    96T
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    CLIA Kit for Rat IL-18
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    U0064h
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    MSDS:
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    96T
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    CLIA Kit for Rat IL-18
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    U0064c
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    Packing:
    96T
    Polyclonal Antibody for Rat IL-18
    Cat.:
    P0064Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat IL-18
    Cat.:
    P0064Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat IL-18
    Polyclonal Antibody for Rat IL-18
    Polyclonal Antibody for Rat IL-18
    Polyclonal Antibody for Rat IL-18
    Polyclonal Antibody for Rat IL-18
    Cat.:
    P0064Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Monoclonal Antibody for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18
    Protein for Rat IL-18

    R&D Technical Data

    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.1%

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    Recovery

    Recovery was determined by spiking various levels of IL-18 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    96-106

    Plasma

    101

    99-104

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    100-105%

    94-101%

    95-102%

    92-97%

    EDTA plasma(n=5)

    90-95%

    94-106%

    105-107%

    87-98%

    heparin plasma(n=5)

    95-103%

     

    91-99%

    104-107%

    98-105%

     

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    References
    1. 1.
      "Induction of interferon-gamma inducing factor in the adrenal cortex."
      Conti B. , Jahng J.W. , Tinti C. , Son J.H. , Joh T.H.
      J. Biol. Chem.272:2035-2037(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA)
      strain: Sprague-Dawley.
      tissue: Adrenal gland.
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA)
      strain: Sprague-Dawley.
      tissue: Brain.
    3. 3.
      "Cloning of the cDNA for interleukin-18 in PC12 and expression in Escherichia coli."
      Kim S.-J. , Kim C.-S. , Song K.-Y. , Kim J.-S. , Jung K.-S.
      Submitted (2003-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA)
      strain: New England Deaconess Hospital.
      tissue: Adrenal gland.
    4. 4.
      "IL-18 translational inhibition restricts IFN-gamma expression in crescentic glomerulonephritis."
      Garcia G.E. , Xia Y. , Ku G. , Johnson R.J. , Wilson C.B. , Feng L.
      Kidney Int.64:160-169(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA)
      strain: Fischer 344.
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